Microbiome Sequencing Solutions
Accurate. Scalable. Research-Driven.
High-Quality 16S rRNA Gene Sequencing & Advanced Bioinformatics Services
Interested in working with us?
"*" indicates required fields
What is 16S rRNA Gene Sequencing?
This approach enables:
- Microbiome profiling
- Taxonomic classification from the phylum to the species level
- Community diversity analysis
- Comparative microbial studies
Our Services
We offer flexible, modular services tailored to your project needs. And you receive structured, publication-ready outputs with interpretable reports.
DNA Extraction:
High-quality extraction protocols optimized for a wide range of sample types.
DNA Quantification:
Accurate DNA measurement to ensure optimal library preparation and sequencing performance.
Library Preparation:
Expert preparation of 16S amplicon libraries for high-throughput sequencing.
16S rRNA Gene Sequencing:
Performed on the Illumina MiSeq platform, with up to 384 samples sequenced per run.
Bioinformatics Analysis:
Our lab provides comprehensive downstream analysis using:
- QIIME2 for data processing
- Amplicon Sequence Variant (ASV) determination
- Taxonomic classification using the Greengenes2 (2022.10) database
- Alpha diversity metrics
- Beta diversity matrices
- Pathway analysis
The Benefits of Illumina MiSeq Technology
We utilize the Illumina MiSeq platform for reliable, high-throughput 16S sequencing with exceptional accuracy and read quality.
- Up to 384 samples per run
- Consistent, reproducible results
- Optimized workflows for microbiome studies
Turnaround Time*: Library Preparation: 4–6 weeks
— Add 2 weeks for: DNA Extraction & Bioinformatics Analysis
*Timelines may vary based on project scope and sample volume.
Pricing
*Up to 384 samples can be sequenced per run.
Why Work With Us?
Submit your project information, and our team will provide a detailed quote and consultation.
Frequently Asked Questions
Can you define the DNA extraction kits you have?
QIAamp Fast DNA Stool Mini Kit – Uses: high-density microbial environments (Examples: fecal, rumen, colonic, and ileal samples). The QIAamp Fast DNA Stool Mini kit enables researchers to perform rapid purification of high-quality genomic DNA from either fresh or frozen gastrointestinal samples from a variety of species. Our lab uses a hybrid protocol that combines enzymatic and mechanical cell degradation to maximize both DNA quality and quantity. For enzymatic degradation, the novel InhibitEX Buffer is used to streamline the removal of PCR inhibitors commonly found in gastrointestinal samples. For mechanical disruption, the MP Bio Lysing Matrix E tubes are utilized to aid in disrupting the peptidoglycan layer in Gram-positive bacterial cell walls, which are usually found in high abundance in gastrointestinal samples.
QIAamp BiOstic Bacteremia DNA Kit – Uses: low density microbial environments (Examples: oral, uterine, milk, respiratory, and blood samples). The QIAamp BiOstic Bacteremia DNA kit provides fast and efficient DNA purification from a variety of samples. This kit removes PCR inhibitors commonly found in blood, swabs, and culture mediums using QIAGEN’s Inhibitor Removal Technology. The kit includes mechanical bead beating lysis resulting in pure genomic DNA isolated that is free of any PCR inhibitors. The resulting isolated DNA is ready to use in a variety of sequencing methods including next-generation sequencing.
Can you explain your DNA quantification process?
Could you outline the process/steps your lab uses for 16S rRNA Gene Sequencing?
Can you tell me more about your bioinformatics service?
Our lab also offers bioinformatic services to be performed on samples following sequencing services. QIIME2 is utilized for all data processing. Additionally, amplicon sequence variants (ASV) are determined using the Greengenes2 2022.10 database. In addition to receiving taxonomic information ranging from the phylum to species level, our bioinformatic analysis provides both alpha and beta diversity matrices and pathway analyses.
Can you explain the benefits of the sequencing platform, Illumina MiSeq?
The Illumina MiSeq is a benchtop sequencer offering high accuracy and versatility for various sequencing needs. Using Illumina’s sequencing by synthesis (SBS) technology, it reads DNA fragments base-by-base, producing reliable data with lower error rates. It provides a relatively quick turnaround, especially for targeted sequencing, microbial genomics, and small-genome de novo sequencing. The MiSeq has flexibility in read length and output, supporting single-read or paired-end reads, which is useful for both short and longer sequences. Its run time can range from a few hours to about a day, depending on the application and read length. The system is compatible with many library prep protocols, enabling applications in amplicon sequencing, 16S rRNA microbiome profiling, and small whole-genome sequencing.
Contact Us
"*" indicates required fields